Gt(ROSA)26Sor^{tm1(Actb-Polb*L22P)Dkid}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm1(Actb-Polb*L22P)Dkid}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 1, Dawit Kidane
• MGI ID: MGI:7550435
• Synonyms: L22P^flox
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm1(Actb-Polb*L22P)Dkid} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:340207
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6 and 129

Mutation description

• Allele Type: Targeted (Conditional ready, Inserted expressed sequence)
• Mutation: Insertion
• Mutation details: A beta-actin promoter and loxP-flanked TK-Neo STOP cassette followed by the Polb gene containing a leucine to proline substitution at amino acid 22 (p.L22P, CTC to CCC) were inserted into the locus. This mutation results in loss of 5-deoxyribose-5-phosphate lyase activity and inefficient base excision repair. The p.L22P variant is associated with gastric cancer in humans. This line is generated from ES clone 89. (J:340207)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 1089 strains or lines available

References

• Original: J:340207 Zhao S, et al., Mutation in DNA Polymerase Beta Causes Spontaneous Chromosomal Instability and Inflammation-Associated Carcinogenesis in Mice. Cancers (Basel). 2019 Aug 13;11(8)
• All: 2 reference(s)