Gt(ROSA)26Sor^{tm2(CAG-Egfr*L860R)Rnem}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm2(CAG-Egfr*L860R)Rnem}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 2, Raphael A Nemenoff
• MGI ID: MGI:7545201
• Synonyms: Egfr^L860R
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm2(CAG-Egfr*L860R)Rnem} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:333112
• Parent Cell Line: JM8A3 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd

Mutation description

• Allele Type: Targeted (Conditional ready, Humanized sequence, Inserted expressed sequence, Reporter)
• Mutations: Insertion, Nucleotide substitutions
• Gt(ROSA)26Sor^{tm2(CAG-Egfr*L860R)Rnem} expresses 1 gene
  Knock-in expresses:
  

  Organism	Expressed Gene	Note
  mouse	Egfr (MGI:95294)	L860R mutation

• Mutation details: The targeting cassette includes (from 5' to 3') a CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter (CAG), a loxP-flanked STOP cassette including neomycin sequence, Egfr mutant cDNA (L860R mutation), mCerulean3 (mC3) sequence, a woodchuck hepatitis virus post-transcriptional regulatory element (WPRE; to enhance the mRNA transcript stability), and polyA signal. This targeting vector was inserted into intron 1 of the Gt(ROSA)26Sor locus. The murine L860R mutation corresponds to the human L858R mutation. (J:333112)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 945 strains or lines available

References

• Original: J:333112 Kleczko EK, et al., Novel EGFR-mutant mouse models of lung adenocarcinoma reveal adaptive immunity requirement for durable osimertinib response. Cancer Lett. 2023 Mar 1;556:216062
• All: 1 reference(s)