Gt(ROSA)26Sortm2(CAG-GshF*)Brsy
Targeted Allele Detail
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Symbol: |
Gt(ROSA)26Sortm2(CAG-GshF*)Brsy |
Name: |
gene trap ROSA 26, Philippe Soriano; targeted mutation 2, Kivanc Birsoy |
MGI ID: |
MGI:7545057 |
Synonyms: |
mito-GshF |
Gene: |
Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
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Alliance: |
Gt(ROSA)26Sortm2(CAG-GshF*)Brsy page
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Germline Transmission: |
Earliest citation of germline transmission:
J:101977
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Parent Cell Line: |
CY2.4 (ES Cell)
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Strain of Origin: |
B6(Cg)-Tyrc-2J
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Allele Type: |
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Targeted (Conditional ready, Epitope tag, Inserted expressed sequence) |
Mutation: |
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Insertion
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Mutation details: The targeting construct containing from 5' to 3': a CAG promoter, a loxP-STOP-loxP, a tandem mitochondrial targeting sequences (MTS) consisting of the Lactobacillus brevis COXIV and the Homo sapiens ACO2 MTS as well as a FLAG tag epitope, and the Streptococcus thermophilus-derived bifunctional glutathione synthetase (mito-GshF) cDNA followed by a stop codon (TAA) was inserted into the Gt(ROSA)26Sor locus. There also is a neomycin cassette in the construct.
(J:101977)
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Original: |
J:101977 The Jackson Laboratory, Information obtained from The Jackson Laboratory, Bar Harbor, ME. Unpublished. 2005-2017; |
All: |
1 reference(s) |
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