Gt(ROSA)26Sor^{tm2(CAG-GshF*)Brsy}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm2(CAG-GshF*)Brsy}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 2, Kivanc Birsoy
• MGI ID: MGI:7545057
• Synonyms: mito-GshF
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm2(CAG-GshF*)Brsy} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:363440
• Parent Cell Line: CY2.4 (ES Cell)
• Strain of Origin: B6(Cg)-Tyr^c-2J

Mutation description

• Allele Type: Targeted (Conditional ready, Epitope tag, Inserted expressed sequence)
• Mutation: Insertion
• Mutation details: The targeting construct containing from 5' to 3': a CAG promoter, a loxP-STOP-loxP, a tandem mitochondrial targeting sequences (MTS) consisting of the Lactobacillus brevis COXIV and the Homo sapiens ACO2 MTS as well as a FLAG tag epitope, and the Streptococcus thermophilus-derived bifunctional glutathione synthetase (mito-GshF) cDNA followed by a stop codon (TAA) was inserted into the Gt(ROSA)26Sor locus. There also is a neomycin cassette in the construct. (J:363440)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 1084 strains or lines available

References

• Original: J:363440 Timson RC, et al., Development of a mouse model expressing a bifunctional glutathione-synthesizing enzyme to study glutathione limitation in vivo. J Biol Chem. 2024 Jan 11;300(2):105645
• All: 1 reference(s)