Tc(HSA19*)SDkn
Targeted Allele Detail

Summary

• Symbol: Tc(HSA19*)SDkn
• Name: transchromosomal, human 19, line VL,William Gottschalk
• MGI ID: MGI:7539501
• Synonyms: APOE-TOMM40S
• Gene: Tc(HSA19*)SDkn Location: unknown Genetic Position: Chr7, Syntenic
• Alliance: Tc(HSA19*)SDkn page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:341425
• Parent Cell Line: C2 (Nagy) (ES Cell)
• Strain of Origin: C57BL/6NTac

Mutation description

• Allele Type: Targeted (Humanized sequence, Inserted expressed sequence)
• Mutations: Insertion, Intergenic deletion
• Tc(HSA19*)SDkn involves 2 genes/genome features (Apoe, Tomm40) View all
• Tc(HSA19*)SDkn expresses 2 genes
  Knock-in expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  APOE (348)

  Apoe	(MGI:88057)

  human

  TOMM40 (10452)

  Tomm40

  (MGI:1858259)

• Mutation details: The allele is created in a multi-step process by exchanging approx. 20 kb on mouse chromosome 7 stretching from and including Tomm40 to Apoe from BAC RP24-136M6 with an acceptor cassette containing PGK-hygromycin and HSV-TK flanked by loxP/lox2272 sites using C57BL/6N-derived C2(Nagy) embryonic stem cells (ES). The 5' loxP site is located downstream of Pvrl2 and the 3'; lox2272 site is located downstream of the last Apoe exon (including the enhancer segment). ES cells carrying the acceptor cassette were electroporated with the exchange vectors and a plasmid carrying Cre recombinase to insert 23.77 kbp from human BAC (RP11-84C16) containing TOMM40 to APOE and the flanking sequences (including promoter and regulatory regions) followed by an FRT-flanked PGK-neo cassette and a loxP site on chr19 : 45,393,394-45,417,163 (HSA19). The modified BAC carries the APOE episilon 3 backbone and the TOMM40 523 polyT short allele (S, T=15). APOE epsilon4 is converted to APOE epsilon3 by exchanging the APOE epsilon4 arginine (CGC) at SNP rs429358 with the APOE epsilon3 cysteine (TGC). (J:341425)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Tc(HSA19*)SDkn Mutation: 1 strain or line available

References

• Original: J:341425 Gottschalk WK, et al., The APOE-TOMM40 Humanized Mouse Model: Characterization of Age, Sex, and PolyT Variant Effects on Gene Expression. J Alzheimers Dis. 2023;94(4):1563-1576
• All: 1 reference(s)