Gt(ROSA)26Sor^{em1(EEF1A1-APX1*)Ykozo}
Endonuclease-mediated Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{em1(EEF1A1-APX1*)Ykozo}
• Name: gene trap ROSA 26, Philippe Soriano; endonuclease-mediated mutation 1, Yevgenia Kozorovitskiy
• MGI ID: MGI:7529474
• Synonyms: APEX2 reporter, DIO- APEX2.NES- P2A- EGFP
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{em1(EEF1A1-APX1*)Ykozo} page

Mutation origin

• Strain of Origin: C57BL/6J

Mutation description

• Allele Type: Endonuclease-mediated (Conditional ready, Epitope tag, Reporter)
• Mutation: Insertion
• Mutation details: CRISPR/cas9 genome editing is used to insert a human EEF1a1 promoter followed by::Lox2272::LoxP::EGFP (inverted)::P2A::APEX-NES (inverted)::FLAG tag::Lox2272::LoxP::hGH double floxed inverted orientation cassette into intron 1 of Gt(ROSA)26Sor. APEX2 is an engineered monomeric peroxidase reporter derived from dimeric soybean ascorbate peroxidase. (J:340416)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 944 strains or lines available

References

• Original: J:340416 Dumrongprechachan V, et al., Dynamic proteomic and phosphoproteomic atlas of corticostriatal axons in neurodevelopment. Elife. 2022 Oct 14;11:e78847
• All: 1 reference(s)