Igs7^{em1(CAG-mCherry)Fcam}
Endonuclease-mediated Allele Detail

Summary

• Symbol: Igs7^{em1(CAG-mCherry)Fcam}
• Name: intergenic site 7; endonuclease-mediated mutation 1, Fernando Camargo
• MGI ID: MGI:7526267
• Synonyms: TA
• Gene: Igs7 Location: unknown Genetic Position: Chr9, Syntenic
• Alliance: Igs7^{em1(CAG-mCherry)Fcam} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:341616
• Parent Cell Line: JM8A3 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd

Mutation description

• Allele Type: Endonuclease-mediated (Lineage barcode, Reporter)
• Mutation: Insertion
• Mutation details: The targeting construct contains the mCherry reporter open reading frame with a WPRE and the 10 target sequences (see below) inserted into the 3 UTR driven by a CAG promoter and inserted into the Igs7 locus using CRISPR/cas9 genome editing. A "barcode" 276 bp array containing 10 target sites in a unique arrangement is inserted upstream of the EGFP polyadenylation site. (J:341616)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Igs7 Mutation: 64 strains or lines available

References

• Original: J:341616 Li L, et al., A mouse model with high clonal barcode diversity for joint lineage, transcriptomic, and epigenomic profiling in single cells. Cell. 2023 Oct 12
• All: 1 reference(s)