Gt(ROSA)26Sor^{tm2.1(CAG-H2BC11)Shna}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm2.1(CAG-H2BC11)Shna}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 2.1, Shinichi Nakagawa
• MGI ID: MGI:7525300
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm2.1(CAG-H2BC11)Shna} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:287786
• Parent Cell Line: TT2 (ES Cell)
• Strain of Origin: (C57BL/6NCrlj x CBA/JNCrlj)F1

Mutation description

• Allele Type: Targeted (Epitope tag, Inserted expressed sequence)
• Mutation: Insertion
• Gt(ROSA)26Sor^{tm2.1(CAG-H2BC11)Shna} expresses 1 gene
  Knock-in expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  H2BC11 (8970)

  H2bc21

  (MGI:2448415)

• Mutation details: The targeting construct contained the CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), Enterobacteria phage P1 loxP sites, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli Neomycin resistance gene, SV40 polyA, FLAG tag sequence (synthetic), human histone cluster 1, H2bj (HIST1H2BJ), and bovine growth hormone polyA signal. Cre-mediated recombination removed the STOP cassette. (J:287786)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 1089 strains or lines available

References

• Original: J:287786 Mito M, et al., Cell Type-Specific Survey of Epigenetic Modifications by Tandem Chromatin Immunoprecipitation Sequencing. Sci Rep. 2018 Jan 18;8(1):1143
• All: 1 reference(s)