Gt(ROSA)26Sor^{tm57.1(CAG-hop/EGFP)Hze}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm57.1(CAG-hop/EGFP)Hze}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 57.1, Hongkui Zeng
• MGI ID: MGI:7523863
• Synonyms: R26-FSF-YFP
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm57.1(CAG-hop/EGFP)Hze} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:219930
• Parent Cell Line: G4 (ES Cell)
• Strain of Origin: (129S6/SvEvTac x C57BL/6NCrl)F1

Mutation description

• Allele Type: Targeted (Conditional ready, Reporter)
• Mutation: Insertion
• Mutation details: Gt(ROSA)26Sor^{tm57(CAG-hop/EGFP)Hze>} line described in J:219930 was crosed to cre deleter to remove the LSL cassette leaving the CAG-FSF-Jaws-GFP-ER2-WPRE-bGHpA inserted between exons 1 and 2 of the Gt(ROSA)26Sor locus. The hop/EGFP fusion protein (Halo57*K200R*W214F/EGFP or Jaws) is composed of a modified Halobacterium halobium (strain shark)-derived halorhodopsin (Halo57) fused in-frame via a 15 nucleotide linker to the N-terminus of enhanced green fluorescent protein (EGFP). The Halo57 sequence has K200R and W214F mutations to substantially boost photocurrents. An endoplasmic reticulum exporting sequence (ER2) from the Kir2.1 inward rectifier potassium channel gene (amino acids FCYENEV) at the C-terminal of EGFP improves membrane trafficking/prevents aggregation. It is a Flp-dependent reporter with an optogenetic silencer function. (J:219930, J:339474)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 1089 strains or lines available

References

• Original: J:339474 Bohic M, et al., A new Hoxb8FlpO mouse line for intersectional approaches to dissect developmentally defined adult sensorimotor circuits. Front Mol Neurosci. 2023;16:1176823
• All: 2 reference(s)