Gt(ROSA)26Sor^{em1(CAG-Ncre/vvd*,-vvd*/Ccre)Larc}
Endonuclease-mediated Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{em1(CAG-Ncre/vvd*,-vvd*/Ccre)Larc}
• Name: gene trap ROSA 26, Philippe Soriano; endonuclease-mediated mutation 1, Tomoji Mashimo
• MGI ID: MGI:7523854
• Synonyms: PA-Cre KI
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{em1(CAG-Ncre/vvd*,-vvd*/Ccre)Larc} page

Mutation origin

• Strain of Origin: C57BL/6J

Mutation description

• Allele Type: Endonuclease-mediated (Inducible, Recombinase)
• Inducer: blue light exposure
• Mutation: Insertion
• Gt(ROSA)26Sor^{em1(CAG-Ncre/vvd*,-vvd*/Ccre)Larc} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  chicken	CAG

• Mutation details: A 7.0 kb PA-Cre expression plasmid under the control of the CAG promoter was introduced at the Gt(ROSA)26Sor locus using the CRISPR/Cas9 system. PA-Cre consists of a split Cre (59/60) with each fragment complemented with nMag or pMag (respectively) and a nuclear localization sequence (NLS) (CreN59-nMag-NLS and NLS-pMag-CreC60), separated by P2A self-cleaving peptide sequence (2A). The nMag and pMag are part of Magnets protein derived from the fragments of photoreceptor gene product Vivid (VVD) from a filamentous fungi Neurospora crassa. (J:339595)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	CAG (chicken) Summary of all recombinase alleles driven by CAG.

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 944 strains or lines available

References

• Original: J:339595 Yoshimi K, et al., Photoactivatable Cre knock-in mice for spatiotemporal control of genetic engineering in vivo. Lab Invest. 2021 Jan;101(1):125-135
• All: 1 reference(s)