Foxi3^em1Akg
Endonuclease-mediated Allele Detail

Summary

• Symbol: Foxi3^em1Akg
• Name: forkhead box I3; endonuclease-mediated mutation 1, Andrew K Groves
• MGI ID: MGI:7522248
• Synonyms: Foxi3^GFP
• Gene: Foxi3 Location: Chr6:70933590-70938050 bp, + strand Genetic Position: Chr6, 32.14 cM
• Alliance: Foxi3^em1Akg page

Mutation origin

• Strain of Origin: C57BL/6

Mutation description

• Allele Type: Endonuclease-mediated (Reporter)
• Mutation: Insertion
• Mutation details: CRISPR/cas9 genome editing used guide RNAs [CCC CCGGGATGGCTCTGATATA] to insert an GSG-P2A peptide sequence and mVenus fluorescent protein coding sequence into the 3' UTR. Foxi3 transcript Foxi3-201 (ENSMUST00000069634.6) was used as reference for the exon number and guide sequences. Donor DNA contained two nucleotide changes (C to T and C to G) to prevent the inserted sequence from being targeted by the Foxi3 gRNA. (J:339365)

Expression

In Mice Carrying this Mutation:

36 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Foxi3 Mutation: 20 strains or lines available

References

• Original: J:339365 Ankamreddy H, et al., Foxi3(GFP) and Foxi3(CreER) mice allow identification and lineage labeling of pharyngeal arch ectoderm and endoderm, and tooth and hair placodes. Dev Dyn. 2023 Aug 6
• All: 2 reference(s)