Pmel^{em1(rtTA,tetO-icre)Osaw}
Endonuclease-mediated Allele Detail

Summary

• Symbol: Pmel^{em1(rtTA,tetO-icre)Osaw}
• Name: premelanosome protein; endonuclease-mediated mutation 1, Masatake Osawa
• MGI ID: MGI:7522089
• Synonyms: Pmel^{P2A-TetON3G-TRE3G-iCre}
• Gene: Pmel Location: Chr10:128540064-128556107 bp, + strand Genetic Position: Chr10, 77.13 cM
• Alliance: Pmel^{em1(rtTA,tetO-icre)Osaw} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:339313
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Endonuclease-mediated (Inducible, Recombinase, Transactivator)
• Inducer: doxycycline/tetracycline
• Mutation: Insertion
• Pmel^{em1(rtTA,tetO-icre)Osaw} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Pmel (MGI:98301)

• Mutation details: The P2A-TetON3G-TRE3G-iCre cassette was inserted into the Pmel locus utilizing the CRISPR/Cas9 in an extended pluripotent stem cell (EPSC) line (C57BL/6J background). (J:339313)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Pmel (mouse) Summary of all recombinase alleles driven by Pmel. Comparative matrix view of recombinase activities Alliance mouse Pmel gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Pmel Mutation: 36 strains or lines available

Notes

Lentiviral gene trap injected into zygotes.

References

• Original: J:339313 Nasrin M, et al., Generation of Pmel-dependent conditional and inducible Cre-driver mouse line for melanocytic-targeted gene manipulation. Pigment Cell Melanoma Res. 2023 Jan;36(1):53-70
• All: 1 reference(s)