Gt(ROSA)26Sor^{em1(CAG-Atoh1/EGFP)Akg}
Endonuclease-mediated Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{em1(CAG-Atoh1/EGFP)Akg}
• Name: gene trap ROSA 26, Philippe Soriano; endonuclease-mediated mutation 1, Andrew K Groves
• MGI ID: MGI:7521997
• Synonyms: Rosa26^Atoh1, Rosa-A
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{em1(CAG-Atoh1/EGFP)Akg} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:339290
• Parent Cell Line: AB2.2 (ES Cell)
• Strain of Origin: 129S7/SvEvBrd-Hprt1^b-m2

Mutation description

• Allele Type: Endonuclease-mediated (Conditional ready, Inserted expressed sequence, Reporter)
• Mutation: Insertion
• Gt(ROSA)26Sor^{em1(CAG-Atoh1/EGFP)Akg} expresses 1 gene
  Knock-in expresses:
  

  Organism	Expressed Gene	Note
  mouse	Atoh1 (MGI:104654)	fused to EGFP

• Mutation details: The targeting vector is designed using a modified Ai3 (Gt(ROSA)26So(tm3(CAG-EYFP)Hze)) vector where the eGFP sequence was replaced with coding regions for atonal bHLH transcription factor 1 (Atoh1) fused to an enhanced green fluorescent protein (eGFP). Specifically, the targeting vector includes (from 5' to 3') a splice acceptor, a CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter (CAG), a loxP-flanked STOP (with stop codons in all 3 reading frames and a triple polyA signal) cassette, Atoh1/eGFP fusion gene, a woodchuck hepatitis virus post-transcriptional regulatory element (WPRE), polyA sequence, followed by an attB/attP-flanked PGK-neo-PGKpA cassette. This entire construct was inserted between exons 1 and 2 of the Gt(ROSA)26Sor locus. (J:339290)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 944 strains or lines available

References

• Original: J:339290 Iyer AA, et al., Cellular reprogramming with ATOH1, GFI1, and POU4F3 implicate epigenetic changes and cell-cell signaling as obstacles to hair cell regeneration in mature mammals. Elife. 2022 Nov 29;11
• All: 1 reference(s)