Pax7^tm1.1Pstu
Targeted Allele Detail

Summary

• Symbol: Pax7^tm1.1Pstu
• Name: paired box 7; targeted mutation 1.1, Pascal Stuelsatz
• MGI ID: MGI:7521966
• Synonyms: Pax7^{tm13955(T2A-ZsGreen)Tac}, Pax7-ZsGreen1
• Gene: Pax7 Location: Chr4:139464373-139560839 bp, - strand Genetic Position: Chr4, 70.83 cM
• Alliance: Pax7^tm1.1Pstu page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:339277
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6NTac

Mutation description

• Allele Type: Targeted (Reporter)
• Mutation: Insertion
• Mutation details: The targeting construct contains the Pax7 open reading frame (ORF), a Kozak sequence to allow protein translation initiation. An FRT-flanked puromycin cassette inserted downstream of exon 8 followed by exon 9, a T2A self-cleaving peptide, ZsGreen1 fluorescent reporter sequence, and 3 nuclear localization signals (3xNLS) inserted upstream of the 3' untranslated region (UTR), Flp-mediated recombination removed the puromyxin cassette. (J:339277)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Pax7 Mutation: 37 strains or lines available

References

• Original: J:339277 Ancel S, et al., A Dual Color Pax7 and Myf5 In Vivo Reporter to Investigate Muscle Stem Cell Heterogeneity in Regeneration and Aging. bioRxiv. 2023
• All: 1 reference(s)