Prm1^em3Ssha
Endonuclease-mediated Allele Detail

Summary

• Symbol: Prm1^em3Ssha
• Name: protamine 1; endonuclease-mediated mutation 3, Saher Sue Hammoud
• MGI ID: MGI:7520863
• Synonyms: P1^delta46-51
• Gene: Prm1 Location: Chr16:10614196-10614687 bp, - strand Genetic Position: Chr16, 5.87 cM, cytoband cen-C3
• Alliance: Prm1^em3Ssha page

Mutation origin

• Strain of Origin: C57BL/6N

Mutation description

• Allele Type: Endonuclease-mediated (Dominant negative)
• Mutation: Intragenic deletion
• Mutation details: CRISPR/Cas9 technology a truncated protein in which residues 46-51 in the C-terminus are deleted. Truncated protein expression is stable but RNA levels are reduced. Incorporation into chromatin occurs but at lower efficiency. (J:338968)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Prm1 Mutation: 7 strains or lines available

References

• Original: J:338968 Moritz L, et al., Sperm chromatin structure and reproductive fitness are altered by substitution of a single amino acid in mouse protamine 1. Nat Struct Mol Biol. 2023 Jul 17
• All: 1 reference(s)