Prm1^em1Ssha
Endonuclease-mediated Allele Detail

Summary

• Symbol: Prm1^em1Ssha
• Name: protamine 1; endonuclease-mediated mutation 1, Saher Sue Hammoud
• MGI ID: MGI:7520861
• Synonyms: P1^K49A
• Gene: Prm1 Location: Chr16:10614196-10614687 bp, - strand Genetic Position: Chr16, 5.87 cM, cytoband cen-C3
• Alliance: Prm1^em1Ssha page

Mutation origin

• Strain of Origin: C57BL/6N

Mutation description

• Allele Type: Endonuclease-mediated (Not Applicable)
• Mutation: Nucleotide substitutions
• Mutation details: CRISPR/Cas9 technology generated an AAA to GCA change resulting in a lusine to alanine substitution at amino acid 49 (p.K49A). A few synonymous alterations were incorporated to introduce an FspI site for genotyping. The K49A mutation results in loss of positive charge as well as loss of acetylation at the site. (J:338968)

Expression

In Mice Carrying this Mutation:

1 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Prm1 Mutation: 5 strains or lines available

References

• Original: J:338968 Moritz L, et al., Sperm chromatin structure and reproductive fitness are altered by substitution of a single amino acid in mouse protamine 1. Nat Struct Mol Biol. 2023 Jul 17
• All: 1 reference(s)