Gt(ROSA)26Sor^{em2(CAG-PACS1)Gath}
Endonuclease-mediated Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{em2(CAG-PACS1)Gath}
• Name: gene trap ROSA 26, Philippe Soriano; endonuclease-mediated mutation 1, Gary Thomas
• MGI ID: MGI:7518817
• Synonyms: Gt(ROSA)26Sor^{tm1(CAG-LSL-Pacs1)Gath}, R26^P1, ROSA2-LSL-PACS1-WT
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{em2(CAG-PACS1)Gath} page

Mutation origin

• Strain of Origin: C57BL/6J

Mutation description

• Allele Type: Endonuclease-mediated (Conditional ready, Epitope tag, Humanized sequence, Inserted expressed sequence)
• Mutation: Insertion
• Gt(ROSA)26Sor^{em2(CAG-PACS1)Gath} expresses 1 gene
  Knock-in expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  PACS1 (55690)

  Pacs1

  (MGI:1277113)

• Mutation details: CRISPR/cas9 endonuclease-mediated genome editing is used to insert hemagluttin-tagged human PACS1 cDNA, followed by an IRES and a GFP reporter under the control of the widespread CAG promoter into the Gt(ROSA)26Sor locus. A loxP-flanked neomycin STOP precedes the PACS1 sequence. The GFP is too low to be detected and was only used for PCR genotyping. (J:338950)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 944 strains or lines available

References

• Original: J:338950 Thomas G, et al., RNA-targeted therapy corrects neuronal deficits in PACS1 syndrome mice. Res Sq. 2023 Jan 27
• All: 1 reference(s)