Sirt1^em1Dahe
Endonuclease-mediated Allele Detail

Summary

• Symbol: Sirt1^em1Dahe
• Name: sirtuin 1; endonuclease-mediated mutation 1, Daniel Herranz
• MGI ID: MGI:7518811
• Synonyms: Sirt1^cKI-H355A
• Gene: Sirt1 Location: Chr10:63154784-63174814 bp, - strand Genetic Position: Chr10, 32.57 cM
• Alliance: Sirt1^em1Dahe page

Mutation origin

• Strain of Origin: C57BL/6N

Mutation description

• Allele Type: Endonuclease-mediated (Conditional ready)
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: CRISPR/cas9 endonuclease-mediated genome editing is used to insert a loxP-flanked Sirt1 minigene that includes exons 5-9, the Sirt1 3' UTR and a STOP into intron 4 of the gene. In addition, a histidine to alanine amino acid substitution at codon 355 (H355A, CAT to GCT) is inserted into the endogenous exon 5. The mutation creates a catalytically dead allele. (J:338949)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Sirt1 Mutation: 81 strains or lines available

References

• Original: J:338949 Lancho O, et al., A Therapeutically Targetable NOTCH1-SIRT1-KAT7 Axis in T-cell Leukemia. Blood Cancer Discov. 2023 Jan 6;4(1):12-33
• All: 1 reference(s)