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Sirt1em1Dahe
Endonuclease-mediated Allele Detail
Summary
Symbol: Sirt1em1Dahe
Name: sirtuin 1; endonuclease-mediated mutation 1, Daniel Herranz
MGI ID: MGI:7518811
Synonyms: Sirt1cKI-H355A
Gene: Sirt1  Location: Chr10:63154784-63174814 bp, - strand  Genetic Position: Chr10, 32.57 cM
Alliance: Sirt1em1Dahe page
Mutation
origin
Strain of Origin:  C57BL/6N
Mutation
description
Allele Type:    Endonuclease-mediated (Conditional ready)
Mutations:    Insertion, Nucleotide substitutions
 
Mutation detailsCRISPR/cas9 endonuclease-mediated genome editing is used to insert a loxP-flanked Sirt1 minigene that includes exons 5-9, the Sirt1 3' UTR and a STOP into intron 4 of the gene. In addition, a histidine to alanine amino acid substitution at codon 355 (H355A, CAT to GCT) is inserted into the endogenous exon 5. The mutation creates a catalytically dead allele. (J:338949)
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Sirt1 Mutation:  81 strains or lines available
References
Original:  J:338949 Lancho O, et al., A Therapeutically Targetable NOTCH1-SIRT1-KAT7 Axis in T-cell Leukemia. Blood Cancer Discov. 2023 Jan 6;4(1):12-33
All:  1 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
03/18/2025
MGI 6.24
The Jackson Laboratory