Vip^{em1(tTA2)Meim}
Endonuclease-mediated Allele Detail

Summary

• Symbol: Vip^{em1(tTA2)Meim}
• Name: vasoactive intestinal polypeptide; endonuclease-mediated mutation 1, Michihiro Mieda
• MGI ID: MGI:7517039
• Synonyms: Vip^tTA
• Gene: Vip Location: Chr10:5589218-5597617 bp, + strand Genetic Position: Chr10, 1.97 cM, cytoband A1
• Alliance: Vip^{em1(tTA2)Meim} page

Mutation origin

• Strain of Origin: C57BL/6J

Mutation description

• Allele Type: Endonuclease-mediated (Transactivator)
• Mutation: Insertion
• Mutation details: CRISPR/cas9-mediated recombination using guide RNA inserted a tTA2-polyA cassette at the start codon in the second exon of the gene. (J:338875)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Vip Mutation: 23 strains or lines available

References

• Original: J:338875 Peng Y, et al., Cell Type-Specific Genetic Manipulation and Impaired Circadian Rhythms in Vip (tTA) Knock-In Mice. Front Physiol. 2022;13:895633
• All: 2 reference(s)