Dnmt1^tm2.1Jae
Targeted Allele Detail

Summary

• Symbol: Dnmt1^tm2.1Jae
• Name: DNA methyltransferase 1; targeted mutation 2.1, Rudolf Jaenisch
• MGI ID: MGI:7516675
• Gene: Dnmt1 Location: Chr9:20818501-20871084 bp, - strand Genetic Position: Chr9, 7.66 cM
• Alliance: Dnmt1^tm2.1Jae page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:236382
• Parent Cell Line: J1 (ES Cell)
• Strain of Origin: 129S4/SvJae

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Homologous recombination inserted a floxed CMV-hygromycin-thymidine kinase expression cassette downstream of exon 5 and placed a loxP site in intron 3. Cre-mediated recombination removed the selection cassette and exons 4 and 5. (J:236382)

Expression

In Mice Carrying this Mutation:

2 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Dnmt1 Mutation: 112 strains or lines available

References

• Original: J:236382 Hargan-Calvopina J, et al., Stage-Specific Demethylation in Primordial Germ Cells Safeguards against Precocious Differentiation. Dev Cell. 2016 Oct 10;39(1):75-86
• All: 1 reference(s)