Rtf1^{tm1c(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Rtf1^{tm1c(KOMP)Wtsi}
• Name: RTF1, Paf1/RNA polymerase II complex component; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:7511793
• Synonyms: Rtf1^flox
• Gene: Rtf1 Location: Chr2:119505549-119565888 bp, + strand Genetic Position: Chr2, 59.97 cM, cytoband E5
• Alliance: Rtf1^{tm1c(KOMP)Wtsi} page
• IMPC: Rtf1 gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:338311
• Parent Cell Line: JM8.F6 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion
• Mutation details: The L1L2_gt0 cassette was inserted at position 119531110 of Chromosome 2 upstream of the critical exon 3 (Build GRCm39). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of exon 3 at position 119531201. Exon 3 is thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele to remove the lacZ sequence and neo selection cassette, leaving loxP sites flanking the critical exon. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:338311)

Disease models

Expression

In Structures Affected by this Mutation:

5 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Rtf1 Mutation: 52 strains or lines available

References

• Original: J:338311 Langenbacher AD, et al., Rtf1 Transcriptionally Regulates Neonatal and Adult Cardiomyocyte Biology. J Cardiovasc Dev Dis. 2023 May 20;10(5)
• All: 1 reference(s)