Ctcf^tm2.2Gal
Targeted Allele Detail

Summary

• Symbol: Ctcf^tm2.2Gal
• Name: CCCTC-binding factor; targeted mutation 2.2, Niels Galjart
• MGI ID: MGI:7509779
• Synonyms: Ctcf^f
• Gene: Ctcf Location: Chr8:106363200-106409554 bp, + strand Genetic Position: Chr8, 53.04 cM
• Alliance: Ctcf^tm2.2Gal page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:101977
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Conditional ready, Reporter)
• Mutation: Insertion
• Mutation details: The targeting vector was designed to insert a loxP-flanked PMC1-neomycin cassette upstream of exon 3 and a loxP-flanked PGK-puromycin cassette followed by a splice acceptor sequence and a lacZ reporter downstream of exon 12. ES cells were isolated de novo from these Ctcf conditional mice, and infected with lentivirus expressing cre recombinase to remove floxed-neo and floxed-puro. ES cells with a single loxP upstream of an exon 3 and another single loxP after the exon 12 essentially flanking the exons 3-12 with lacZ reporter downstream, were selected. (J:143271)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Ctcf Mutation: 77 strains or lines available

References

• Original: J:143271 Heath H, et al., CTCF regulates cell cycle progression of alphabeta T cells in the thymus. EMBO J. 2008 Nov 5;27(21):2839-50
• All: 3 reference(s)