Gt(ROSA)26Sor^{tm1(AKT1*E17K)Maib}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm1(AKT1*E17K)Maib}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 1, Maike Buchner
• MGI ID: MGI:7509229
• Synonyms: AKT1^E17K, Gt(ROSA)26Sor^{tm1(AKT1*E17K,EGFP)Maib}
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm1(AKT1*E17K)Maib} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:307913
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129

Mutation description

• Allele Type: Targeted (Conditional ready, Humanized sequence, Inserted expressed sequence)
• Mutation: Insertion
• Gt(ROSA)26Sor^{tm1(AKT1*E17K)Maib} expresses 1 gene
  Knock-in expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  AKT1 (207)

  Akt1	(MGI:87986)

  contains p.E17K mutation

• Mutation details: The following were inserted into intron 1 of the locus: splice acceptor sequence, a loxP site flanked neomycin resistance gene cassette and STOP cassette, a modified human AKT1 cDNA (where a G-to-A point mutation changed glutamic acid codon 17 to lysine (p.E17K) and codon-optimized for mouse expression), IRES sequence, the EGFP reporter gene, and poly(A) signal sequences. The inserted sequence is only expressed after Cre-mediated deletion of the neo-STOP cassette. (J:307913)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 1089 strains or lines available

References

• Original: J:307913 Ecker V, et al., Targeted PI3K/AKT-hyperactivation induces cell death in chronic lymphocytic leukemia. Nat Commun. 2021 Jun 10;12(1):3526
• All: 1 reference(s)