Gt(ROSA)26Sor^{em2(CAG-Tbx2,-Atoh1,-tdTomato)Zyliu}
Endonuclease-mediated Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{em2(CAG-Tbx2,-Atoh1,-tdTomato)Zyliu}
• Name: gene trap ROSA 26, Philippe Soriano; endonuclease-mediated mutation 2, Zhiyong Liu
• MGI ID: MGI:7495873
• Synonyms: Rosa26-CAG-Loxp-Stop-Loxp-Tbx2*3xV5-P2A-DHFR-Atoh1*3xHA-DHFR-T2A-Tdtomato, Rosa26-LSL-TAT
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{em2(CAG-Tbx2,-Atoh1,-tdTomato)Zyliu} page

Mutation origin

• Strain of Origin: (C57BL/6 x DBA/2J)F1

Mutation description

• Allele Type: Endonuclease-mediated (Conditional ready, Epitope tag, Inserted expressed sequence, Reporter)
• Mutation: Insertion
• Gt(ROSA)26Sor^{em2(CAG-Tbx2,-Atoh1,-tdTomato)Zyliu} expresses 2 genes
  Knock-in expresses:
  

  Organism	Expressed Gene	Note
  mouse	Atoh1 (MGI:104654)
  mouse	Tbx2 (MGI:98494)

• Gt(ROSA)26Sor^{em2(CAG-Tbx2,-Atoh1,-tdTomato)Zyliu} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  chicken	CAG

• Mutation details: CRISPR/cas9 genome editing is used to insert the following from 5' to 3': a CAG promoter, a lox-stop-lox (LSL), a long cistronic DNA element containing Tbx2 fused with three V5 fragments, a P2A sequence, an Atoh1 fused with 3 hemaglutinin tags (HA) flanked by 5' and 3' DHFR (E. Coli dihydrofolate reductase) destabilizing domains, a T2A site and a Tdtomato reporter followed by a WPRE polyA sequence inserted in downstream of exon 1. The DHFR domains flanking Atoh1 create an unstable protein that undergoes rapid degradation in the absence of trimethoprin (TMP). (J:337553)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 1062 strains or lines available

References

• Original: J:337553 Bi Z, et al., Development and transdifferentiation into inner hair cells require Tbx2. Natl Sci Rev. 2022 Dec;9(12):nwac156
• All: 2 reference(s)