Epas1^{em1(EPAS1_iL)Eez}
Endonuclease-mediated Allele Detail

Summary

• Symbol: Epas1^{em1(EPAS1_iL)Eez}
• Name: endothelial PAS domain protein 1; endonuclease-mediated mutation 1, Eric E Zhang
• MGI ID: MGI:7494257
• Gene: Epas1 Location: Chr17:87061292-87140838 bp, + strand Genetic Position: Chr17, 56.9 cM
• Alliance: Epas1^{em1(EPAS1_iL)Eez} page

Mutation origin

• Strain of Origin: B6.129S6-Per2^tm1Jt/J

Mutation description

• Allele Type: Endonuclease-mediated (Epitope tag, Inserted expressed sequence)
• Mutation: Insertion
• Mutation details: CRISPR/Cas9 technology inserted the coding sequence of the plateau pika (Ochotona curzoniae) long form EPAS1, 3xFLAG tag 6XHis tag, TAG, SV40 and polyA signal after the start codon of the first exon. Only the inserted pika protein, and not the mouse protein, is expressed. (J:325224)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Epas1 Mutation: 66 strains or lines available

References

• Original: J:325224 Liu N, et al., A highland-adaptation mutation of the Epas1 protein increases its stability and disrupts the circadian clock in the plateau pika. Cell Rep. 2022 May 17;39(7):110816
• All: 1 reference(s)