Tnfaip3^em1Mpa
Endonuclease-mediated Allele Detail

Summary

• Symbol: Tnfaip3^em1Mpa
• Name: tumor necrosis factor, alpha-induced protein 3; endonuclease-mediated mutation 1, Manolis Pasparakis
• MGI ID: MGI:7484798
• Synonyms: A20^mZnF7
• Gene: Tnfaip3 Location: Chr10:18876658-18891158 bp, - strand Genetic Position: Chr10, 8.08 cM
• Alliance: Tnfaip3^em1Mpa page

Mutation origin

• Strain of Origin: C57BL/6

Mutation description

• Allele Type: Endonuclease-mediated (Not Applicable)
• Mutation: Nucleotide substitutions
• Mutation details: CRISPR/Cas9 technology targeting the ZnF7 domain generated two cysteine to alanine point mutations at residues 764 and 767: TGC to GCC (p.C764A) and TGC to GCT (p.C767A). Two silent mutations [GCC to GCT (A758) and TAC to TAT (Y768)] were also introduced to avoid re-editing. These mutations abrogate binding ability to polyubiquitin chains. (J:282944)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Tnfaip3 Mutation: 44 strains or lines available

References

• Original: J:282944 Polykratis A, et al., A20 prevents inflammasome-dependent arthritis by inhibiting macrophage necroptosis through its ZnF7 ubiquitin-binding domain. Nat Cell Biol. 2019 Jun;21(6):731-742
• All: 2 reference(s)