Egr1^{tm1.1(cre/ERT2)Jglt}
Targeted Allele Detail

Summary

• Symbol: Egr1^{tm1.1(cre/ERT2)Jglt}
• Name: early growth response 1; targeted mutation 1.1, Jean-Antoine Girault
• MGI ID: MGI:7484542
• Synonyms: Egr1-CreER^T2, Tg(Egr1-CreERT2)
• Gene: Egr1 Location: Chr18:34994260-34998009 bp, + strand Genetic Position: Chr18, 18.76 cM, cytoband C/D
• Alliance: Egr1^{tm1.1(cre/ERT2)Jglt} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:335757
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6N

Mutation description

• Allele Type: Targeted (Inducible, Recombinase)
• Inducer: tamoxifen
• Mutation: Insertion
• Egr1^{tm1.1(cre/ERT2)Jglt} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Egr1 (MGI:95295)

• Mutation details: A targeting vector consisting of a Cre/ERT2 cassette and a neomycin resistance gene flanked by FRT sequences were inserted into the transcription start site of the Egr1 gene in a bacterial artificial chromosome (BAC, clone RP23-70M6) that contained the entire mouse Egr1 gene with 95 kb 5 upstream and 86 kb downstream regions was used for homologous recombination in the ES cells. After selection and cloning of the ES cells, the neomycin resistance gene was removed by flipase and the resulting transgenic ES cells were injected into agouti blastocysts to obtain chimeric mice. Two lines (U62-2 and U62-7) were obtained, and U62-2 line was established and used for the studies. (J:335757)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Egr1 (mouse) Summary of all recombinase alleles driven by Egr1. Comparative matrix view of recombinase activities Alliance mouse Egr1 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Egr1 Mutation: 19 strains or lines available

References

• Original: J:335757 Longueville S, et al., Long-lasting tagging of neurons activated by seizures or cocaine administration in Egr1-CreER(T2) transgenic mice. Eur J Neurosci. 2021 Mar;53(5):1450-1472
• All: 3 reference(s)