Cdkn1c^{em2(cre/ERT2)Kei}
Endonuclease-mediated Allele Detail

Summary

• Symbol: Cdkn1c^{em2(cre/ERT2)Kei}
• Name: cyclin dependent kinase inhibitor 1C; endonuclease-mediated mutation 2, Keiichi I Nakayama
• MGI ID: MGI:7482648
• Synonyms: p57-CreER^T2
• Gene: Cdkn1c Location: Chr7:143012076-143014735 bp, - strand Genetic Position: Chr7, 88.22 cM
• Alliance: Cdkn1c^{em2(cre/ERT2)Kei} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:323000
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Endonuclease-mediated (Inducible, Recombinase)
• Inducer: tamoxifen
• Mutation: Insertion
• Cdkn1c^{em2(cre/ERT2)Kei} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Cdkn1c (MGI:104564)

• Mutation details: Using CRISPR/Cas9 system, the T2A-cre/ERT2 cassette was inserted in-frame into the endogenous Cdkn1c (p57) coding sequence immediately 5 to the only TGA codon at this locus in ES cells. Single colony ES cell clones that had undergone homologous recombination were then expanded and injected into C57BL/6 mouse blastocysts to generate chimeric mice. (J:323000)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Cdkn1c (mouse) Summary of all recombinase alleles driven by Cdkn1c. Comparative matrix view of recombinase activities Alliance mouse Cdkn1c gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cdkn1c Mutation: 19 strains or lines available

Notes

Lentiviral gene trap injected into zygotes.

References

• Original: J:323000 Higa T, et al., Spatiotemporal reprogramming of differentiated cells underlies regeneration and neoplasia in the intestinal epithelium. Nat Commun. 2022 Mar 21;13(1):1500
• All: 2 reference(s)