Csf2^tm1.1Lky
Targeted Allele Detail

Summary

• Symbol: Csf2^tm1.1Lky
• Name: colony stimulating factor 2 (granulocyte-macrophage); targeted mutation 1.1, Richard M. Locksley
• MGI ID: MGI:7482635
• Synonyms: Csf2^fl, Csf2^RFP
• Gene: Csf2 Location: Chr11:54138097-54140493 bp, - strand Genetic Position: Chr11, 32.13 cM
• Alliance: Csf2^tm1.1Lky page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:323412
• Parent Cell Line: PRX-B6T (ES Cell)
• Strain of Origin: C57BL/6J

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change, Reporter)
• Mutation: Insertion
• Mutation details: The targeting construct inserted a loxP site upstream of exon 3 and loxP site, IRES, tdTomato reporter gene, and FRT-flanked neomycin resistance cassette downstream of exon 4. Flp-mediated recombination removed the selection cassette. tdTomato is expressed both in the presence and absence of recombination of the loxP sites flanking exons 3 and 4. (J:323412)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Csf2 Mutation: 28 strains or lines available

References

• Original: J:323412 Gschwend J, et al., Alveolar macrophages rely on GM-CSF from alveolar epithelial type 2 cells before and after birth. J Exp Med. 2021 Oct 4;218(10):e20210745
• All: 3 reference(s)