Igs7^{tm229(tetO-GCaMP6m,-ChRmine*,SYN1-tTA2)Daigl}
Targeted Allele Detail

Summary

• Symbol: Igs7^{tm229(tetO-GCaMP6m,-ChRmine*,SYN1-tTA2)Daigl}
• Name: intergenic site 7; targeted mutation 229, Tanya Daigle
• MGI ID: MGI:7482008
• Synonyms: Ai229, Ai229^{TIT2L-GCaMP6m-IRES2-ChRmine-TS-Kv2.1-HA-WPRE-ISL-tTA2-WPRE_hyg}, Igs7^{tm229(tetO-GCaMP6m,-ChRmine*,SYN1-tTA2)Tasic}
• Gene: Igs7 Location: unknown Genetic Position: Chr9, Syntenic
• Alliance: Igs7^{tm229(tetO-GCaMP6m,-ChRmine*,SYN1-tTA2)Daigl} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:101977
• Parent Cell Line: G4 (ES Cell)
• Strain of Origin: (129S6/SvEvTac x C57BL/6NCrl)F1

Mutation description

• Allele Type: Targeted (Conditional ready, Epitope tag, Inducible, Reporter, Transactivator)
• Mutation: Insertion
• Mutation details: The targeting vector has a Tet response element (tetO), loxP-STOP-loxP cassette, GCaMP6m-IRES2-ChRmine-TS-Kv2.1-HA-WPRE, a CAG promoter, Lox2272-STOP-Lox2272 cassette, ChRmine* sequence, and then a fragment of the human synapsin 1 (SYN1) gene promoter, LoxN-STOP-LoxN cassette, and tTA2-WPRE sequence- all inserted into the T1 TIGRE locus (an intergenic region on chromosome 9 [between AB124611 and Carm1] determined to have low basal transcriptional activity and allow high Cre and/or Tet inducibility). ChRmine is a pump-like cation-conducting channelrhodopsin, derived from Rhodomonas lens, that exhibits extremely high current and light sensitivity, as well as a markedly red-shifted spectrum. GCaMP7m is an ultrasensitive EGFP fluorescent calcium detector of neuronal activity with medium decay and response kinetics. Of note, the Ai229 allele was designed with an IRES upstream of the ChRmine* sequence to reduce/eliminate leakiness in the absence of Cre recombinase. (J:101977)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Igs7 Mutation: 70 strains or lines available

References

• Original: J:101977 The Jackson Laboratory, Information obtained from The Jackson Laboratory, Bar Harbor, ME. Unpublished. 2005-2017
• All: 1 reference(s)