Igs7^{tm218(tetO-rho*/mNeonGreen,CAG-mRuby3,-tTA2)Daigl}
Targeted Allele Detail

Summary

• Symbol: Igs7^{tm218(tetO-rho*/mNeonGreen,CAG-mRuby3,-tTA2)Daigl}
• Name: intergenic site 7; targeted mutation 218, Tanya Daigle
• MGI ID: MGI:7482002
• Synonyms: Ai218, Ai218^{TITL-Ace2N-4AA-mNeonGreen-TS-ER-WPRE-ICL-mRuby3-IRES2-tTA2-WPRE}, Igs7^{tm218(tetO-rho*/mNeonGreen,CAG-mRuby3,-tTA2)Tasic}
• Gene: Igs7 Location: unknown Genetic Position: Chr9, Syntenic
• Alliance: Igs7^{tm218(tetO-rho*/mNeonGreen,CAG-mRuby3,-tTA2)Daigl} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:370610
• Parent Cell Line: G4 (ES Cell)
• Strain of Origin: (129S6/SvEvTac x C57BL/6NCrl)F1

Mutation description

• Allele Type: Targeted (Conditional ready, Inducible, Reporter, Transactivator)
• Mutation: Insertion
• Mutation details: The targeting vector has a Tet response element (tetO), loxP-STOP-loxP cassette and a Ace2N-4AA-mNeonGreen-WPRE sequence that is followed by a CAG promoter, Lox2272-STOP-Lox2272 cassette, mRuby3, and IRES-tTA2-WPRE sequence - all inserted into the T1 TIGRE locus (an intergenic region on chromosome 9 [between AB124611 and Carm1] determined to have low basal transcriptional activity and allow high cre and/or Tet inducibility). Ace2N (rho*)-4AA-mNeonGreen is an indicator variant of Acetabularia acetabulum-derived rhodopsin (Ace) with an inactivated proton pump (Ace2N), with four extra amino acids in a linker domain that fuses to mNeonGreen fluorescent protein to enable voltage-sensitive fluorescence resonance energy transfer (FRET). Of note, the Ai218 allele was designed with an IRES upstream of the tTA2 to reduce/eliminate leakiness in the absence of cre recombinase. (J:101977, J:370610)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Igs7 Mutation: 76 strains or lines available

References

• Original: J:370610 Haziza S, et al., Imaging high-frequency voltage dynamics in multiple neuron classes of behaving mammals. Cell. 2025 Aug 7;188(16):4401-4423.e31
• All: 2 reference(s)