Igs7^{tm218(tetO-rho*/mNeonGreen,CAG-mRuby3,-tTA2)Tasic}
Targeted Allele Detail

Summary

• Symbol: Igs7^{tm218(tetO-rho*/mNeonGreen,CAG-mRuby3,-tTA2)Tasic}
• Name: intergenic site 7; targeted mutation 218, Bosiljka Tasic
• MGI ID: MGI:7482002
• Synonyms: Ai218, Ai218^{TITL-Ace2N-4AA-mNeonGreen-TS-ER-WPRE-ICL-mRuby3-IRES2-tTA2-WPRE}
• Gene: Igs7 Location: unknown Genetic Position: Chr9, Syntenic
• Alliance: Igs7^{tm218(tetO-rho*/mNeonGreen,CAG-mRuby3,-tTA2)Tasic} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:101977
• Parent Cell Line: G4 (ES Cell)
• Strain of Origin: (129S6/SvEvTac x C57BL/6NCrl)F1

Mutation description

• Allele Type: Targeted (Conditional ready, Inducible, Reporter, Transactivator)
• Mutation: Insertion
• Mutation details: The targeting vector has a Tet response element (tetO), loxP-STOP-loxP cassette and a Ace2N-4AA-mNeonGreen-WPRE sequence that is followed by a CAG promoter, Lox2272-STOP-Lox2272 cassette, mRuby3, and IRES-tTA2-WPRE sequence - all inserted into the T1 TIGRE locus (an intergenic region on chromosome 9 [between AB124611 and Carm1] determined to have low basal transcriptional activity and allow high cre and/or Tet inducibility). Ace2N (rho*)-4AA-mNeonGreen is an indicator variant of Acetabularia acetabulum-derived rhodopsin (Ace) with an inactivated proton pump (Ace2N), with four extra amino acids in a linker domain that fuses to mNeonGreen fluorescent protein to enable voltage-sensitive fluorescence resonance energy transfer (FRET). Of note, the Ai218 allele was designed with an IRES upstream of the tTA2 to reduce/eliminate leakiness in the absence of cre recombinase. (J:101977)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Igs7 Mutation: 64 strains or lines available

References

• Original: J:101977 The Jackson Laboratory, Information obtained from The Jackson Laboratory, Bar Harbor, ME. Unpublished. 2005-2017
• All: 1 reference(s)