Igs7^{tm203(tetO-sChroME*/GCaMP7s,CAG-mRuby3,-tTA2)Tasic}
Targeted Allele Detail

Summary

• Symbol: Igs7^{tm203(tetO-sChroME*/GCaMP7s,CAG-mRuby3,-tTA2)Tasic}
• Name: intergenic site 7; targeted mutation 203, Bosiljka Tasic
• MGI ID: MGI:7482000
• Synonyms: Ai203, Ai203^{TITL-sChroME-Kv2.1-GC7s-WPRE-ICL-nls-mRuby3-IRES2-tTA2-WPRE}
• Gene: Igs7 Location: unknown Genetic Position: Chr9, Syntenic
• Alliance: Igs7^{tm203(tetO-sChroME*/GCaMP7s,CAG-mRuby3,-tTA2)Tasic} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:339333
• Parent Cell Line: G4 (ES Cell)
• Strain of Origin: (129S6/SvEvTac x C57BL/6NCrl)F1

Mutation description

• Allele Type: Targeted (Conditional ready, Inducible, Reporter, Transactivator)
• Mutation: Insertion
• Mutation details: The targeting vector has a Tet response element (tetO), loxP-STOP-loxP cassette and a sChroME*/GCaMP7s-WPRE sequence that is followed by a CAG promoter, Lox2272-STOP-Lox2272 cassette, mRuby3, and IRES-tTA2-WPRE sequence - all inserted into the T1 TIGRE locus (an intergenic region on chromosome 9 [between AB124611 and Carm1] determined to have low basal transcriptional activity and allow high cre and/or Tet inducibility). sChroME*/GCaMP7s is the result of a sChroME* coupled to the fluorescent reporter GCaMP7s (an ultrasensitive EGFP fluorescent calcium detector of neuronal activity with slow decay and response kinetics). sChroME is a soma-targeted (ST) ultrafast and highly potent opsin with 3-5x larger photocurrents than opsins commonly used for multiphoton optogenetics. Of note, the Ai203 allele was designed with an IRES upstream of the tTA2 to reduce/eliminate leakiness in the absence of cre recombinase. (J:339333)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Igs7 Mutation: 64 strains or lines available

References

• Original: J:339333 Bounds HA, et al., All-optical recreation of naturalistic neural activity with a multifunctional transgenic reporter mouse. Cell Rep. 2023 Aug 4;42(8):112909
• All: 1 reference(s)