Fgf8^{tm3.1(cre/Esr1*)Moon}
Targeted Allele Detail

Summary

• Symbol: Fgf8^{tm3.1(cre/Esr1*)Moon}
• Name: fibroblast growth factor 8; targeted mutation 3.1, Anne M Moon
• MGI ID: MGI:7471382
• Synonyms: Fgf8^mcm
• Gene: Fgf8 Location: Chr19:45725237-45731354 bp, - strand Genetic Position: Chr19, 38.75 cM, cytoband C3-D
• Alliance: Fgf8^{tm3.1(cre/Esr1*)Moon} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:301046
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Inducible, Recombinase)
• Inducer: tamoxifen
• Mutation: Insertion
• Fgf8^{tm3.1(cre/Esr1*)Moon} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Fgf8 (MGI:99604)

• Mutation details: The targeting construct inserted an IRES sequence followed by the mER:Cre:mER cDNA encoding a fusion protein with two copies of the mouse estrogen receptor (mER) flanking Cre recombinase, and a Neomycin resistance (NeoR) selection cassette flanked by Frt recombination sites into an Eag1 site in the Fgf8 gene located 36 bp 3 of the Fgf8 stop codon and 5 to the endogenous polyadenylation sequence. The linearized targeting vector was electroporated into mouse embryonic stem cells and subjected to selection. Flp-recombinase excised the NeoR. (J:301046)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Fgf8 (mouse) Summary of all recombinase alleles driven by Fgf8. Comparative matrix view of recombinase activities Alliance mouse Fgf8 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Fgf8 Mutation: 18 strains or lines available

References

• Original: J:232111 Emechebe U, et al., T-box3 is a ciliary protein and regulates stability of the Gli3 transcription factor to control digit number. Elife. 2016;5:e07897
• All: 3 reference(s)