Gt(ROSA)26Sor^tm8Sor
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^tm8Sor
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 8, Philippe Soriano
• MGI ID: MGI:7467457
• Synonyms: SAT
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^tm8Sor page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:339772
• Parent Cell Line: AK7 (ES Cell)
• Strain of Origin: 129S4/SvJaeSor

Mutation description

• Allele Type: Targeted (Conditional ready, Reporter)
• Mutation: Insertion
• Mutation details: A viral splice acceptor (SA)-loxP-neomycin-pA-loxP-FOXO1-mRuby3-P2A-H2B-mTagBFP2 (BFP)-P2A-ERK-KTR-mClover3-WPRE-pA cassette is introduced to the mouse Gt(ROSA)26Sor locus. The targeting vectorincludes DNA fragments contained mClover3 (Addgene, #(a href="https://www.addgene.org/74252/" target="_blank")74252(/a)), mRuby3 (Addgene, #(a href="https://www.addgene.org/74252/" target="_blank")74252(/a)), mTagBFP2 (Addgene, #(a href="https://www.addgene.org/99267/" target="_blank")99267(/a); BFP), human H2B (Addgene, #(a href="https://www.addgene.org/99267/" target="_blank")99267(/a)), an adenovirus splice acceptor, a floxed NeoR sequence, the ERK-KTR reporter, the FOXO1 reporter, and woodchuck hepatitis virus post-transcriptional regulatory element (from ROSA26(Tdt) mouse genomic DNA. The ERK-KTR sensor consists of an ERK1/2 binding site and phosphorylation motif adjacent to phosphorylation activated nuclear export signal (NES) and inhibited nuclear localization sequence (NLS) fused to a fluorescent protein (FP; mClover3). The AKT sensor uses a mutant version of FOXO1 with defective DNA binding and relies on endogenous AKT phosphorylation sites that regulate its nuclear localization. (J:339772)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 1084 strains or lines available

References

• Original: J:339772 Dinsmore CJ, et al., Conditional fluorescent mouse translocation reporters for ERK1/2 and AKT signaling. Dev Biol. 2023 Sep 1;503:113-119
• All: 1 reference(s)