Dll4^{em1(cre/ERT2)Bz}
Endonuclease-mediated Allele Detail

Summary

• Symbol: Dll4^{em1(cre/ERT2)Bz}
• Name: delta like canonical Notch ligand 4; endonuclease-mediated mutation 1, Bin Zhou
• MGI ID: MGI:7467077
• Synonyms: Dll4^creERT2
• Gene: Dll4 Location: Chr2:119156265-119166147 bp, + strand Genetic Position: Chr2, 59.97 cM, cytoband E3
• Alliance: Dll4^{em1(cre/ERT2)Bz} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:317975
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Endonuclease-mediated (Inducible, Recombinase)
• Inducer: tamoxifen
• Mutation: Insertion
• Dll4^{em1(cre/ERT2)Bz} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Dll4 (MGI:1859388)

• Mutation details: The allele was generated by knocking in a 2A peptide-linked creERT2 cDNA immediately before the stop codon of the Dll4 gene by CRISPRCas9 targeting and homozygous recombination in R1 mouse embryonic stem cells. (J:317975)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Dll4 (mouse) Summary of all recombinase alleles driven by Dll4. Comparative matrix view of recombinase activities Alliance mouse Dll4 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Dll4 Mutation: 26 strains or lines available

References

• Original: J:317975 Lu P, et al., Perinatal angiogenesis from pre-existing coronary vessels via DLL4-NOTCH1 signalling. Nat Cell Biol. 2021 Sep;23(9):967-977
• All: 1 reference(s)