Gt(ROSA)26Sor^{tm10.1(CAG-hop/EYFP)Imayo}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm10.1(CAG-hop/EYFP)Imayo}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 10.1, Itaru Imayoshi
• MGI ID: MGI:7464520
• Synonyms: Tg(Gt(ROSA)26Sor-eNpHR3/EYFP)1.1Imayo
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm10.1(CAG-hop/EYFP)Imayo} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:104881
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6

Mutation description

• Allele Type: Targeted (Reporter)
• Mutation: Insertion
• Mutation details: The transgene targeted to the locus contains a splice acceptor flanked puro cassette, CAG promoter, floxed stop cassette, FRT-flanked stop cassette, the halorhodopsin from the halophilic bacterium Natronomas pharaonis modified with an N-terminal signal peptide and a C-terminal endoplasmic reticulum export sequence fused in-frame with an enhanced yellow fluorescent protein (eNpHR3-EYFP), and a woodchuck hepatitis virus post-transcriptional regulatory element (WPRE; to enhance the mRNA transcript stability). Flp-mediated recombination removed the FRT-flanked stop cassette. (J:104881)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 1062 strains or lines available

References

• Original: J:104881 The RIKEN BioResource Center, Information obtained from The RIKEN BioResource Center. Unpublished. 2006-2013
• All: 1 reference(s)