Gt(ROSA)26Sor^{tm1(CAG-Thy1,-srta)Vic}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm1(CAG-Thy1,-srta)Vic}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 1, Gabriel Victora
• MGI ID: MGI:7464413
• Synonyms: Rosa26^uLIPSTIC
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm1(CAG-Thy1,-srta)Vic} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:354843
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6

Mutation description

• Allele Type: Targeted (Conditional ready, Epitope tag, Inserted expressed sequence)
• Mutation: Insertion
• Gt(ROSA)26Sor^{tm1(CAG-Thy1,-srta)Vic} expresses 1 gene
  Knock-in expresses:
  

  Organism	Expressed Gene	Note
  mouse	Thy1 (MGI:98747)	fused to a G5 tag

• Mutation details: The targeting vector is constructed based on the Ai9 vector from Addgene with (from 5' to 3') a CAG (CMV enhancer/chicken beta-actin) promoter, an FRT site, a loxP site, a mouse CD40 signal peptide, a myc tag, a G5 tag (five N-terminal glycine residues) fused to Thy1.1 cDNA, a polyA signal, a second loxP site, a FLAG tag, a Staphylococcus aureus transpeptidase sortase A (srta)-tag cDNA attached to a linker and a human PDGFRB transmembrane domain followed by a woodchuck hepatitis virus post-transcriptional regulatory element (WPRE), a polyA signal, and an attB/attP-flanked PGK-FRT-Neo-polyA cassette. (J:334846, J:354843)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 1084 strains or lines available

References

• Original: J:354843 Nakandakari-Higa S, et al., Universal recording of immune cell interactions in vivo. Nature. 2024 Mar;627(8003):399-406
• All: 3 reference(s)