Gt(ROSA)26Sor^em1Crx
Endonuclease-mediated Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^em1Crx
• Name: gene trap ROSA 26, Philippe Soriano; endonuclease-mediated mutation 1, Colin Ross
• MGI ID: MGI:7461949
• Synonyms: LumA
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^em1Crx page

Mutation origin

• Strain of Origin: C57BL/6J

Mutation description

• Allele Type: Endonuclease-mediated (Reporter)
• Mutation: Insertion
• Mutation details: A guide RNA [5'- GCAGATCACGAGGGAAGAGG-3'] is selected to introduce a full-length luciferase gene, containing the R387X (c.A1159T) nonsense mutation and T381T silent mutation, into the Gt(ROSA)26Sor locus. The silent T381T mutation creates a new PAM site required for the binding of the base editor. (J:334636)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 956 strains or lines available

References

• Original: J:334636 Yu SY, et al., A luciferase reporter mouse model to optimize in vivo gene editing validated by lipid nanoparticle delivery of adenine base editors. Mol Ther. 2023 Apr 5;31(4):1159-1166
• All: 1 reference(s)