About   Help   FAQ
Endonuclease-mediated Allele Detail
Symbol: Sema4fem1(IMPC)J
Name: sema domain, immunoglobulin domain (Ig), TM domain, and short cytoplasmic domain; endonuclease-mediated mutation 1, Jackson
MGI ID: MGI:7447135
Gene: Sema4f  Location: Chr6:82888865-82916724 bp, - strand  Genetic Position: Chr6, 35.94 cM
Alliance: Sema4fem1(IMPC)J page
IMPC: Sema4f gene page
Strain of Origin:  C57BL/6NJ
Project Collection: IMPC
Allele Type:    Endonuclease-mediated (Null/knockout)
Mutation:    Intragenic deletion
Mutation detailsThis allele was generated at The Jackson Laboratory by electroporating Cas9 protein and 2 guide sequences TTGGTAGTCACAGTCACGCG and TTCTAGGGATGCTTTCATGG, which resulted in a 554 bp deletion beginning at Chromosome 6 position 82,907,725 bp and ending after 82,908,278 bp (GRCm39/mm39). This mutation deletes ENSMUSE00000194640 (exon 5) and 460 bp of flanking intronic sequence including the splice acceptor and donor and is predicted to cause a change of amino acid sequence after residue 158 and early truncation 8 amino acids later. (J:188991)
Inheritance:    Not Specified
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 2 strains available      Cell Lines: 0 lines available
Carrying any Sema4f Mutation:  26 strains or lines available
Original:  J:188991 The Jackson Laboratory, Alleles produced for the KOMP project by The Jackson Laboratory. MGI Direct Data Submission. 2012;
All:  1 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support.
last database update
MGI 6.22
The Jackson Laboratory