Slc25a47^{tm1c(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Slc25a47^{tm1c(EUCOMM)Hmgu}
• Name: solute carrier family 25, member 47; targeted mutation 1c, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:7447020
• Synonyms: Slc25a47flox
• Gene: Slc25a47 Location: Chr12:108815740-108822741 bp, + strand Genetic Position: Chr12, 59.65 cM
• Alliance: Slc25a47^{tm1c(EUCOMM)Hmgu} page
• IMPC: Slc25a47 gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:334152
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion
• Mutation details: The L1L2_GT0_LF2A_LacZ_BetactP_neo cassette was inserted at position 108820794 of Chromosome 12 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 108822998. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele to remove the lacZ sequence and neo selection cassette, leaving loxP sites flanking the critical exon(s). Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:334152)

Expression

In Mice Carrying this Mutation:

1 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Slc25a47 Mutation: 15 strains or lines available

References

• Original: J:334152 Bresciani N, et al., The Slc25a47 locus is a novel determinant of hepatic mitochondrial function implicated in liver fibrosis. J Hepatol. 2022 Oct;77(4):1071-1082
• All: 3 reference(s)