Gt(ROSA)26Sor^{tm11(CAG-ZsGreen,CAG-tdTomato)Bzsh}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm11(CAG-ZsGreen,CAG-tdTomato)Bzsh}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 11, Bin Zhou
• MGI ID: MGI:7444982
• Synonyms: Gt(ROSA)26Sor^{tm11(CAG-ZsGreen,-tdTomato)Bzsh}, R26-rGlR, R26-TLR
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm11(CAG-ZsGreen,CAG-tdTomato)Bzsh} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:285035
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Conditional ready, Reporter)
• Mutation: Insertion
• Mutation details: A CAG-rox-stop-rox-ZsGreen-WPRE-pA-Frt-Neo-Frt sequence into exon1 of the Rosa26 locus and the insulator-CAG-loxP-stop-loxP-tdTomato-WPRE-pA sequence into exon2 of the Rosa26 gene locus using traditional targeted mutation through homologous recombination in embryonic stem cells. It is a Dre/rox and Cre/loxP dual reporter. With Dre recombinase activity, the first STOP sequence is excised and ZsGreen can be expressed. With Cre recombinase activity, the second STOP sequence is removed allowing the expression of tdTomato downstream. (J:285035)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 1205 strains or lines available

References

• Original: J:285035 Liu K, et al., Triple-cell lineage tracing by a dual reporter on a single allele. J Biol Chem. 2020 Jan 17;295(3):690-700
• All: 5 reference(s)