Tg(CAG-Katshuka)3Sgo
Transgene Detail

Summary

• Symbol: Tg(CAG-Katshuka)3Sgo
• Name: transgene insertion 3, Sagrario Ortega
• MGI ID: MGI:7444781
• Synonyms: Tg(CAG-LSL-KFP)
• Transgene: Tg(CAG-Katshuka)3Sgo Location: unknown
• Alliance: Tg(CAG-Katshuka)3Sgo page

Transgene origin

• Strain of Origin: (C57BL/6JOlaHsd x CBA/J)F1

Transgene description

• Transgene Type: Transgenic (Conditional ready, Reporter)
• Mutation: Insertion
• Mutation details: The transgenic construct consists of the ubiquitous promoter CAG, composed of the cytomegalovirus (CMV) immediate early enhancer and the modified chicken b-actin promoter, a loxP-flanked transcription STOP cassette (LSL) followed by the cDNA of Katushka fluorescent protein (KFP), and a rabbit beta-globin polyadenylation signal. The Katushka expression is driven by the CAG promoter upon Cre recombinase-mediated excision of the floxed STOP cassette. Five founder mice were generated, three of which transmitted germline. Two lines 3 and 13 which contained a single integration event, were further expanded and characterized. No significant differences were observed between them. Most of the studies were performed with line 3. (J:167974)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:167974 Dieguez-Hurtado R, et al., A Cre-reporter transgenic mouse expressing the far-red fluorescent protein Katushka. Genesis. 2011 Jan;49(1):36-45
• All: 1 reference(s)