Ass1^{tm1c(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Ass1^{tm1c(EUCOMM)Hmgu}
• Name: argininosuccinate synthetase 1; targeted mutation 1c, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:7441939
• Gene: Ass1 Location: Chr2:31360282-31410682 bp, + strand Genetic Position: Chr2, 21.81 cM
• Alliance: Ass1^{tm1c(EUCOMM)Hmgu} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:302898
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion
• Mutation details: The targeting cassette is composed of an FRT site followed by lacZ sequence and an initial loxP site upstream of exon 8. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of exon 8. Flp-mediated recombination removed the lacZ and neo cassette. (J:302898)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Ass1 Mutation: 56 strains or lines available

References

• Original: J:302898 Rogers LC, et al., Intracellular arginine-dependent translation sensor reveals the dynamics of arginine starvation response and resistance in ASS1-negative cells. Cancer Metab. 2021 Jan 21;9(1):4
• All: 2 reference(s)