Comt^tm1.1Tamm
Targeted Allele Detail

Summary

• Symbol: Comt^tm1.1Tamm
• Name: catechol-O-methyltransferase; targeted mutation 1.1, Anne Tammimaki
• MGI ID: MGI:7439774
• Synonyms: MB-COMT^-
• Gene: Comt Location: Chr16:18225636-18245602 bp, - strand Genetic Position: Chr16, 11.4 cM
• Alliance: Comt^tm1.1Tamm page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:333577
• Parent Cell Line: G4 (ES Cell)
• Strain of Origin: (129S6/SvEvTac x C57BL/6NCrl)F1

Mutation description

• Allele Type: Targeted (Modified isoform(s))
• Mutation: Nucleotide substitutions
• Mutation details: A construct containing a modified version of exon 2 with two point mutations (ATGCTG to GAGCTC) in the P2 promoter and a floxed pgk-neo cassette in intron 2 was inserted replacing the endogenous sequence via homologous recombination. One of the point mutations in exon 2 converts methionine to glutamic acid while the second is silent.Cre mediated recombination then removed the neo cassette. The mutant gene expresses only the soluble isoform, expression of the membrane-bound isoform is absent. (J:333577)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Comt Mutation: 23 strains or lines available

References

• Original: J:333577 Tammimaki A, et al., Generation of membrane-bound catechol-O-methyl transferase deficient mice with disctinct sex dependent behavioral phenotype. J Physiol Pharmacol. 2016 Dec;67(6):827-842
• All: 1 reference(s)