Gt(ROSA)26Sor^{tm1(CAG-dcas9,-EGFP)Hfuj}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm1(CAG-dcas9,-EGFP)Hfuj}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 1, Hodaka Fujii
• MGI ID: MGI:7439568
• Synonyms: 3xFLAG-dCas9/CTV (neo+/GFP+), Gt(ROSA)26Sor^{tm1(CAG-3XFLAG/dCas9,-EGFP)Hfuj}, Gt(ROSA)26Sor^{tm1(CAG-cas9*,-EGFP)Hfuj}
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm1(CAG-dcas9,-EGFP)Hfuj} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:258204
• Parent Cell Line: B6JN/1 (ES Cell)
• Strain of Origin: (C57BL/6N x C57BL/6J)F1

Mutation description

• Allele Type: Targeted (Endonuclease, Epitope tag, Reporter)
• Mutation: Insertion
• Mutation details: CRISPR-targeting inserted into the locus a CAG promoter driving expression of a FLAG-tag Streptococcus pyogenes dead Cas9 (dCas9) and FRT-flanked IRES and EGFP reporter gene. A floxed stop cassette prevents expression of the cas9 gene. (J:258204)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 1060 strains or lines available

References

• Original: J:258204 Fujita T, et al., Transgenic mouse lines expressing the 3xFLAG-dCas9 protein for enChIP analysis. Genes Cells. 2018 Apr;23(4):318-325
• All: 1 reference(s)