Sfrp5^{em1.1(ERT2/cre)Kkb}
Endonuclease-mediated Allele Detail

Summary

• Symbol: Sfrp5^{em1.1(ERT2/cre)Kkb}
• Name: secreted frizzled-related sequence protein 5; endonuclease-mediated mutation 1.1, Hiroki Kokubo
• MGI ID: MGI:7437538
• Synonyms: Sfrp5-ERT2Cre
• Gene: Sfrp5 Location: Chr19:42186410-42190691 bp, - strand Genetic Position: Chr19, 35.93 cM, cytoband D1
• Alliance: Sfrp5^{em1.1(ERT2/cre)Kkb} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:245018
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Endonuclease-mediated (Inducible, Recombinase)
• Inducer: tamoxifen
• Mutations: Insertion, Intergenic deletion
• Sfrp5^{em1.1(ERT2/cre)Kkb} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Sfrp5 (MGI:1860298)

• Mutation details: A targeting vector consisting of a cassette containing ERT2-Cre-recombinase genes with frt-PGK-neomycin (neo)-bpA-frt was used for homologous recombination in ES cells using the CRISPR-Cas9 system to replace the exon 1 of the Sfrp5 gene. Frt-neo was then removed by crossing with Rosa-Flp mice. (J:245018)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Sfrp5 (mouse) Summary of all recombinase alleles driven by Sfrp5. Comparative matrix view of recombinase activities Alliance mouse Sfrp5 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Sfrp5 Mutation: 25 strains or lines available

Notes

Lentiviral gene trap injected into zygotes.

References

• Original: J:245018 Fujii M, et al., Sfrp5 identifies murine cardiac progenitors for all myocardial structures except for the right ventricle. Nat Commun. 2017 Mar 13;8:14664
• All: 1 reference(s)