Lztfl1^tm1.3Zpl
Targeted Allele Detail

Summary

• Symbol: Lztfl1^tm1.3Zpl
• Name: leucine zipper transcription factor-like 1; targeted mutation 1.3, Zhi-Ping Liu
• MGI ID: MGI:7435277
• Synonyms: Lztfl1^-, Lztfl1^tm1d
• Gene: Lztfl1 Location: Chr9:123523469-123546690 bp, - strand Genetic Position: Chr9, 74.36 cM, cytoband F
• Alliance: Lztfl1^tm1.3Zpl page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:287261
• Parent Cell Line: JM8 (ES Cell)
• Strain of Origin: C57BL/6N

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The L1L2_Pgk_P cassette L3L4_pD223_DTA_spec backbone from project CSD50165 was used to generate this allele. The cassette is composed of an FRT site followed by a lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the PGK promoter, sV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon 4. The critical exon 4 is thus flanked by loxP sites. Flp-mediated recombination removed the lacZ and neomycin resistance gene. Cre-mediated recombination deleted exon 4. Western blot analysis confirmed absence of protein in brain tissue lysates. (J:287261)

Disease models

Expression

In Structures Affected by this Mutation:

9 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Lztfl1 Mutation: 19 strains or lines available

References

• Original: J:287261 Wei Q, et al., Lztfl1/BBS17 controls energy homeostasis by regulating the leptin signaling in the hypothalamic neurons. J Mol Cell Biol. 2018 Oct 1;10(5):402-410
• All: 1 reference(s)