Macroh2a1^em1Jwji
Endonuclease-mediated Allele Detail

Summary

• Symbol: Macroh2a1^em1Jwji
• Name: macroH2A.1 histone; endonuclease-mediated mutation 1, Jianwei Jiao
• MGI ID: MGI:7433085
• Synonyms: mH2A1.2^-
• Gene: Macroh2a1 Location: Chr13:56221435-56283439 bp, - strand Genetic Position: Chr13, 30.06 cM, cytoband B2
• Alliance: Macroh2a1^em1Jwji page

Mutation origin

• Strain of Origin: ICR

Mutation description

• Allele Type: Endonuclease-mediated (Modified isoform(s))
• Mutation: Intragenic deletion
• Mutation details: CRISPR/Cas9 technology generated a 54,449 bp deletion encompassing exons 1-8. qRT-PCR indicates hardly any transcript expression of the mH2A1.2 isoform and normal transcript levels of the mH2A1.1 isoform. Western blot analysis shows absence of expression in cortices at E13.5. (J:333071)

Expression

In Mice Carrying this Mutation:

21 assay results 1 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Macroh2a1 Mutation: 50 strains or lines available

References

• Original: J:333071 Ma H, et al., MacroH2A1.2 deficiency leads to neural stem cell differentiation defects and autism-like behaviors. EMBO Rep. 2021 Jul 5;22(7):e52150
• All: 1 reference(s)