Gt(ROSA)26Sorem28(KRT18-ACE2)Mvw
Endonuclease-mediated Allele Detail
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Symbol: |
Gt(ROSA)26Sorem28(KRT18-ACE2)Mvw |
Name: |
gene trap ROSA 26, Philippe Soriano; endonuclease-mediated mutation 27, Micheal V Wiles |
MGI ID: |
MGI:7432933 |
Synonyms: |
RMCE[K18-HuACE2] |
Gene: |
Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
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Alliance: |
Gt(ROSA)26Sorem28(KRT18-ACE2)Mvw page
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Allele Type: |
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Endonuclease-mediated (Epitope tag, Humanized sequence, Inserted expressed sequence) |
Mutation: |
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Insertion
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Gt(ROSA)26Sorem28(KRT18-ACE2)Mvw expresses
1 gene
Knock-in expresses:
Organism |
Expressed Gene |
Homolog in Mouse |
Note |
human |
ACE2 (59272) |
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Mutation details: CRISPR/cas9 genome editing and RMCE (recombinase-mediated cassette exchange) are utilized to insert a human keratin 18 promoter directing expression of the full length human ACE2with a C-terminal FLAG epitope tag into a modified Gt(ROSA)26Sor (NSG.RosaBxb-GT/GA) locus. The NOD.Cg-Prkdc Il2rg/SzJ (NSG)-derived zygotes carrying the Gt(ROSA)26Sor (RosaBxb-GT/GA) allele were previously targeted to insert a dual Bxb1 attP-site "safe harbor landing pad" in the first intron of Gt(ROSA)26Sor. The landing pad contains a 48 bp Bxb1 attP-GT sequence and a 48 bp Bxb1 attP-GA sequence separated by a 240 bp spacer of native Gt(ROSA)26Sor sequence. The DNA donor plasmid, carrying two heterologous attB sites and donor transgene, was then microinjected with Bxb1 integrase into the RMCE-ready zygotes (attP-GT/GA). This allele is similar to the em27 allele described in Low BE, et al Sci Rep 2022.
(J:101977)
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Original: |
J:101977 The Jackson Laboratory, Information obtained from The Jackson Laboratory, Bar Harbor, ME. Unpublished. 2005-2017; |
All: |
1 reference(s) |
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