Pf4^tm1Ipek
Targeted Allele Detail

Summary

• Symbol: Pf4^tm1Ipek
• Name: platelet factor 4; targeted mutation 1, Philipp von Hundelshausen
• MGI ID: MGI:7432868
• Synonyms: Cxcl4^L1
• Gene: Pf4 Location: Chr5:90920362-90921242 bp, + strand Genetic Position: Chr5, 44.78 cM
• Alliance: Pf4^tm1Ipek page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:270132
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Conditional ready, Humanized sequence)
• Mutation: Insertion
• Mutation details: The targeting vector replaced exons 1-3 with exon 1, an FRT-flanked neomycin selection cassette, a loxP site, exon 2, exon 3 containing three amino acid changes [substitution of proline at amino acid 64 to leucine (p.P64L), lysine at amino acid 72 to glutamine (p.K72E), and isoleucine at amino acid 73 to histidine (p.I73H)], and a second loxP site. The three amino acid substitutions in exon 2 were introduced to obtain the murine ortholog of human PF4. (J:270132)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Pf4 Mutation: 18 strains or lines available

References

• Original: J:270132 von Hundelshausen P, et al., Chemokine interactome mapping enables tailored intervention in acute and chronic inflammation. Sci Transl Med. 2017 Apr 05;9(384)
• All: 2 reference(s)