Il1a^em1Tdk
Endonuclease-mediated Allele Detail

Summary

• Symbol: Il1a^em1Tdk
• Name: interleukin 1 alpha; endonuclease-mediated mutation 1, Thirumala-Devi Kanneganti
• MGI ID: MGI:7431279
• Synonyms: Il1a-KO^line2
• Gene: Il1a Location: Chr2:129141530-129151892 bp, - strand Genetic Position: Chr2, 62.9 cM, cytoband F
• Alliance: Il1a^em1Tdk page

Mutation origin

• Strain of Origin: Not Applicable

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: CRISPR/cas9 genome editing uses guide RNAs (AAAAGCTTCTGACGTACCACagg and AAGTAACAGCGGAGCGCTTTtgg, PAM sequences indicated by lowercase letters) to delete exons 2 to 6. Il1a transcript from Mus musculus interleukin 1 alpha (Il1a); NCBI Reference Sequence: NM_010554.4, was used as reference for the exon numbering and the guide/donor sequences. (J:332933)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Il1a Mutation: 29 strains or lines available

References

• Original: J:332933 Malireddi RKS, et al., Determining distinct roles of IL-1alpha through generation of an IL-1alpha knockout mouse with no defect in IL-1beta expression. Front Immunol. 2022;13:1068230
• All: 1 reference(s)